Neisseria gonorrhoeae confirmation and antimicrobial susceptibility testing
Which test do we use?
Culture and identification of Neisseria gonorrhoeae (Gram staining and oxidase test) and confirmation with MALDI-TOF test to verify diagnosis of N. gonorrhoeae .
Determination of the MIC (Minimal Inhibitory Concentration determination) by e-test for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin and the agar dilution method for the other antibiotics.
Sampling instructions
A subculture of the isolate will be inoculated on a blood agar culture medium.
After a maximum incubation time of 20 hours at 33-37°C in 5-7% CO 2 , the inoculated blood agar will be sent to the ITM at room temperature (20-25°C). As far as possible, the transport time should be limited to 24 hours.
Please ensure that all samples are identified with a unique number and that the corresponding request form is included in the shipment.
Use the request form.
Storage conditions
Please send the sample immediately to the ITM after a maximum incubation of 20 hours as mentioned above.
Important advice
INACCEPTABLE DEMANDES
Not viable isolates or blood agar medium without visual growth of N. gonorrhoeae growing colonies.
Frequence
The confirmation and identification of N. gonorhoeae: daily.
The antibiotic susceptibility analysis is performed weekly for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin.
The antibiotic susceptibility analyses for the other antibiotics will only be performed 4 x a year (only for surveillance purposes).
Reference values
Not applicable.
Delivery time of the result
The confirmation and identification of N. gonorrhoeae together with the susceptibility results for Azithromycin, Ceftriaxone and Ciprofloxacin will be sent within 7 working days.
The other antibiotic susceptibility analyses will be performed 4 x a year (only for surveillance purposes).
Accredited
Yes
ISO 15189 – BELAC 147-MED
Loinc-code
PCR: 24111-7
Laatste wijziging op: 08/08/2023