Culture and identification of Neisseria gonorrhoeae (Gram staining and oxidase test) and confirmation with a molecular assay to verify diagnosis of N. gonorrhoeae .
Determination of the MIC (Minimal Inhibitory Concentration determination) by e-test for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin and the agar dilution method for the other antibiotics.
A subculture of the isolate will be inoculated on a blood agar culture medium.
After a maximum incubation time of 20 hours at 33-37°C in 5-7% CO 2 , the inoculated blood agar will be sent to the ITM at room temperature (20-25°C). As far as possible, the transport time should be limited to 24 hours.
Please ensure that all samples are identified with a unique number and that the corresponding request form is included in the shipment.
Use the request form.
Please send the sample immediately to the ITM after a maximum incubation of 20 hours as mentioned above.
Not viable isolates or blood agar medium without visual growth of N. gonorrhoeae growing colonies.
The confirmation and identification of N. gonorhoeae: daily.
The antibiotic susceptibility analysis is performed weekly for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin.
The antibiotic susceptibility analyses for the other antibiotics will only be performed 4 x a year (only for surveillance purposes).
The confirmation and identification of N. gonorrhoeae together with the susceptibility results for Azithromycin, Ceftriaxone and Ciprofloxacin will be sent within 7 working days.
The other antibiotic susceptibility analyses will be performed 4 x a year (only for surveillance purposes).
ISO 15189 – BELAC 147-MED
Laatste wijziging op: 09/06/2020