Neisseria gonorrhoeae confirmation and antimicrobial susceptibility testing

Which test do we use?

Culture and identification of Neisseria gonorrhoeae (Gram staining and oxidase test) and confirmation with a molecular assay to verify diagnosis of N. gonorrhoeae .

 

Determination of the MIC (Minimal Inhibitory Concentration determination) by e-test for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin and the agar dilution method for the other antibiotics.

Sampling instructions

A subculture of the isolate will be inoculated on a blood agar culture medium.

After a maximum incubation of 20 hours at 33-37°C in 5-7% CO 2 , the inoculated blood agar will be sent to the ITM at room temperature (20-25°C).

 

Please ensure that all samples are identified with a unique number and that the corresponding request form is included in the shipment.

 

Use the request form.

Storage conditions

Please send the sample immediately to the ITM after a maximum incubation of 20 hours as mentioned above.

Important advice

INACCEPTABLE DEMANDES

 

Not viable isolates or blood agar medium without visual growth of N. gonorrhoeae growing colonies.

Frequence

The confirmation and identification of N. gonorhoeae: daily.

 

The antibiotic susceptibility analysis is performed weekly for the following antibiotics: Azithromycin, Ceftriaxone and Ciprofloxacin.

 

The antibiotic susceptibility analyses for the other antibiotics will only be performed 4 x a year (only for surveillance purposes).

Reference values

Not applicable.

How soon can you expect results?

The confirmation and identification of N. gonorrhoeae together with the susceptibility results for Azithromycin, Ceftriaxone and Ciprofloxacin will be sent within 7 working days.

 

The other antibiotic susceptibility analyses will be performed 4 x a year (only for surveillance purposes).

Accredited

Yes

ISO 15189 – BELAC 147-MED

Laatste wijziging op: 06/09/2019