Mycoplasma genitalium PCR

Which test do we use?

  • Detection of confirmation of Mycoplasma genitalium in genital secretes and urines by real-time PCR.
  • Weekly macrolide resistance determination by Real-time PCR.
  • Trimestrial macrolide and fluoroquinolone resistance determination by Sanger sequencing.

Sampling instructions

Only when non-gonococcal non-chlamydial urethritis or cervicitis is suspected, the following samples can be sent to the ITM.


  • A genital sample collected with a dry synthetic swab or E-swab.
  • Urine.
  • Contact the laboratory for other material.




  • Eswab: with min. 500 µl transport medium.
  • Dry swabs: a dry synthetic swab in a closed container.
  • Urine: min. 5ml.
  • Other material: please contact the laboratory.


Please ensure that all samples are identified with a unique number and that the corresponding request form is included in the shipment.


Use the request form.

Storage conditions

If possible, send the samples as soon as possible to the ITM.


  • Urines can be stored at 2-8°C for max. 4 days.
  • Frozen urines can also be sent to the ITM with the use of cool packs or can be sent frozen on dry ice.
  • Dry swabs can be stored at -18°C and are sent to ITM on dry ice.
  • E-swabs can be stored at 2°C – 30°C for max 4 days and can be sent to the ITM on room temperature.

Important advice

Whenever possible, it is recommended that all samples are shipped immediately after collection.


  • All samples will need to be shipped together with the corresponding request form to the laboratory.
  • If the form is missing, the analysis will not be performed !




  • Swabs with wooden or metal shafts, or with a cotton tip will be rejected.
  • All samples will need to be stored and sent correctly to the ITM according to the described rules.


  • Screenings PCR: 1 x per week.
  • Macrolide resistance determination:
    • Via PCR: 1 x per week
    • Via Sanger sequencing: quarterly
  • Fluoro-quinolone resistance determination via Sanger sequencing: quarterly.

Reference values


Delivery time of the result

Depends on the total number of requested analyses.

See ‘How frequently do we perform this analysis’.


Yes, only for detection and confirmation.

Not for resistance determination.

ISO 15189 – BELAC 147-MED



Laatste wijziging op: 06/09/2023