Mycobacteria identification from culture

Which test do we use?

• AgMPT64 rapid test for identification of M. tuberculosis complex, if acid-fast rods were detected in pure culture.
• If negative for AgMPT64 rapid test and presence of acid-fast rods, further identification follows via DNA extraction followed by an in-house PCR based on the gene encoding 16S-rRNA (rrs), with primers specific for the genus Mycobacterium. Positive amplicons are sequenced for identification of atypical mycobacteria at the species level. This method also identifies the few M. tuberculosis complex isolates that tested false negative on the AgMPT64 rapid test.

Sampling instructions

Possibly on positive cultures with demonstrated acid-fast rods (solid or liquid nutrient media).

Storage conditions

Positive cultures are best stored at room temperature or specific incubation temperature. Positive cultures can be sent at room temperature.

Important advice

For sending cultures on solid nutrient media, the moisture should be removed from the tubes in a sterile manner!