Leptospira antibodies

Which test do we use?

Microscopic agglutination test (MAT) to detect specific antibodies (total Ig). We have a rapid diagnostic IgM test for urgent analyses of acute infections (please mention symptoms and date of onset of symptoms on de request form). The result always has to be confirmed by MAT.

Sampling instructions

For 1 or 2 serological analyses: at least 1 ml serum
For 3 to 5 serological analyses: at least 2 ml serum

Storage conditions

The serum can be sent at room temperature, but the duration of transportation must be reduced as much as possible (avoid weekends or holidays).

Important advice

Antibodies can usually be detected 7 to 10 days after the onset of the disease.

The panel of strains tested has been chosen based on exposure in Belgium. It is of paramount importance that the laboratory receives clinical information and knowledge about the country of exposure to properly interpret the results.

At the early stage of the infection a cross reaction between different strains is to be expected. Later on, the cross-reacting antibodies will disappear faster than the serovar-specific antibodies which can be detected for years after exposure.


4x / week
In case of urgency: daily (urgent requests should be motivated)

Reference values


Delivery time of the result

rapid diagnostic IgM test: day of the request

MAT: 3 business days after delivery sample


MAT: yes
ISO 15189 – Belac 147-MED
IgM: no


551294 / 551305



Laatste wijziging op: 16/01/2023