Real-time PCR for the detection of monkeypox virus
Take a swab of the lesions, preferably in combination with an anorectal swab if the patient is an MSM. It is best to sample more than one lesion, preferably from different locations on the body and/or from lesions that look different.
Other samples that may be collected (depending on the clinical picture) are a swab of mucosal lesions (e.g. in the mouth), an anorectal swab in case of anal complaints, saliva or a throat swab in case of tonsillitis and urine or a urethral swab in case of urethritis. In the prodromal phase, saliva or a throat swab and possibly EDTA whole blood/serum/plasma may be useful.
Preferably use a swab in a tube containing inactivation medium. The following media were validated for MPXV-PCR:
If not available, a skin swab in Universal Transport Medium (UTM) or eSwab can also be sent.
4°C until transport.
2 business days
Laatste wijziging op: 17/01/2023