HIV 1 resistance testing

Which test do we use?

In-house PCR (HIV-1 Protease RT and Integrase)
The sequencing is outsourced to Eurofins

Sampling instructions

  • Adults: at least 9 ml EDTA blood
  • Children under 12 years of age: at least 3,5ml EDTA blood

Samples must not be older than 24 hours after collection

Resistance determination can also be done on the plasma (EDTA) of the HIV viral load

Storage conditions

Blood can be stored and sent at room temperature (20-25°C)
Blood samples should arrive at the laboratory between 8:30am and 3.00 pm.

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Plasma must be kept at -80°C and sent on dry ice.
Plasma samples should arrive at the laboratory between 8:30am and 5.00 pm.

Important advice

External laboratories can prepare the samples according to the procedure (BIJ-0012). Includes packaging instructions for plasma.

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Resistance determination can only be requested by physicians of the AIDS Reference Centre Antwerp (ARC) for patients tracked at the centre. Contact the laboratory in all other cases.

Frequence

On request (usually weekly)

Reference values

Completely sensitive

How soon can you expect results?

Approximately 20 working days

Accredited

Yes (ISO 15189 – 147-MED)(except sequencing at Eurofins – ISO 17025)

Laatste wijziging op: 23/01/2020