HIV 1 resistance testing
Which test do we use?
In-house PCR (HIV-1 Protease RT and Integrase)
The sequencing is outsourced to Eurofins
Sampling instructions
- Adults: at least 9 ml EDTA blood
- Children under 12 years of age: at least 3,5ml EDTA blood
Samples must not be older than 24 hours after collection
Resistance determination can also be done on the plasma (EDTA) sample that was used for the HIV viral load test.
Storage conditions
Blood can be stored and sent at room temperature (20-25°C)
Blood samples should arrive at the laboratory between 8:30am and 3.00 pm.
Plasma must be kept at -80°C and sent on dry ice.
Plasma samples should arrive at the laboratory between 8:30am and 5.00 pm.
Important advice
External laboratories can prepare the samples according to the procedure. Includes packaging instructions for plasma.
Resistance determination can only be requested by physicians of the AIDS Reference Center Antwerp (ARC) for patients tracked at the center. Contact the laboratory in all other cases.
Frequence
On request (usually weekly)
Reference values
Completely sensitive
Delivery time of the result
Approximately 20 business days
Accredited
Yes (ISO 15189 – 147-MED)(except sequencing at Eurofins – ISO 17025)
Laatste wijziging op: 16/01/2023