Micrscopy + serology
once per week
33.50 per test
* Due to the lack of sensitivity (20%) a parasitological request for T.evansi is always combined with an antibody detection test. Mention your VAT number. For EU countries, submission forms lacking a VAT number will be submitted to 21% VAT.
** charged once per request
Preparing a smear
Wipe the slide with alcohol. Handle slides by edges only. (Any grease on the slide will cause the dried blood to flake off during staining.)
Dispense a small drop (5 µl) of blood near one end of a slide. As shown in the picture, bring the edge of another slide in contact with the drop and allow the drop to bank evenly behind the spreader. The angle between the two slides has to be 30-40 degrees. Now, push to the other end of the slide in a smooth, quick motion.
It is important that the volume of blood is not excessive. So, if you succeed in making a gradual transition from thick to thin in your smear, you should get a zone with a satisfactory distribution of cells.
It’s a good idea to prepare several smears. In fact, they aren’t always successful, and with several attempts, your chances of getting a well-prepared one increase.
Let the smears dry at ambient temperature for at leas 30 minutes. Observe the smears with the microscope to check whether they are properly made. The red cells must not overlap each other, nor be so scarce as to be too spread out.
Maternal antibodies may be present until 6 months after birth
Consignee for T.evansi samples
Institute of Tropical Medicine
Applied Technology & Production Unit
Tel: 32 (0)3 247 63 51 or 32 (0)3 247 63 68
For CATT test and microscopic examination urgent testing is possible. An extra costs of 10% will be charged.
CATT serum & microscopic examination
Laatste wijziging op: 18/11/2019