Trypanosoma evansi

Method

Micrscopy + serology

Species

all mammals

Sample specification

serum

freq./delay

once per week

Price*

33.50 per test

Administration cost

50

* Due to the lack of sensitivity (20%) a parasitological request for T.evansi is always combined with an antibody detection test. Mention your VAT number. For EU countries, submission forms lacking a VAT number will be submitted to 21% VAT.

** charged once per request

Which test do we use?

  • Card Agglutination Test (CATT) for antibodies.
  • Immuno trypanolysis (confirmation test for positive CATT)
  • Microscopic examination of a smear after Giemsa staining.
  • PCR for parasite DNA
  • ELISA for antibodies (only Equidae and Camelidae)

Sampling instructions

  • CATT:
    Minimum 1-ml serum
  • Immuno trypanolysis:
    Minimum 1-ml serum or plasma (EDTA/heparin), or 2-ml blood (EDTA/heparin)
  • Microscopic examination
    Unstained good quality smears made from fresh blood
  • PCR
    1-ml blood (EDTA/heparin)
  • ELISA
    Minimum 1-ml serum or plasma (EDTA/heparin), or 2-ml blood (EDTA/heparin)

Storage conditions

  • Serum: -20°C to +30°C
  • Blood: 2-30°C
  • Smears: ambient temperature

Important advice

Preparing a smear

Wipe the slide with alcohol. Handle slides by edges only. (Any grease on the slide will cause the dried blood to flake off during staining.)

Dispense a small drop (5 µl) of blood near one end of a slide. As shown in the picture, bring the edge of another slide in contact with the drop and allow the drop to bank evenly behind the spreader. The angle between the two slides has to be 30-40 degrees. Now, push to the other end of the slide in a smooth, quick motion.

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It is important that the volume of blood is not excessive. So, if you succeed in making a gradual transition from thick to thin in your smear, you should get a zone with a satisfactory distribution of cells.

It’s a good idea to prepare several smears. In fact, they aren’t always successful, and with several attempts, your chances of getting a well-prepared one increase.

Let the smears dry at ambient temperature for at leas 30 minutes. Observe the smears with the microscope to check whether they are properly made. The red cells must not overlap each other, nor be so scarce as to be too spread out.

Serology

Maternal antibodies may be present until 6 months after birth

Consignee for T.evansi samples
Institute of Tropical Medicine
Applied Technology & Production Unit
Nationalestraat 155
B-2000 Antwerp
Tel: 32 (0)3 247 63 51 or 32 (0)3 247 63 68

Information
Practical questions about sampling and shipment: surra@itg.be
Questions about diagnosis and interpretation of results: pbuscher@itg.be
Managament: Diane Jacquet and Philippe Büscher

Aanvraagformulier

Frequence

  • CATT: Once a week on Tuesday
  • Immuno trypanolysis: on demand
  • Microscopic examination: Once a week (≥10 slides delivery delay 2 to 3 weeks)
  • PCR: on demand
  • ELISA: on demand

Reference values

  • CATT: positive as from 1/4 (exception: canine positive as from 1/8)
  • Immuno trypanolysis: Percent lysis 0%
  • Microscopic examination: No trypanosomes detected.
  • PCR:
  • ELISA: Percent Positivity < 30%

How soon can you expect results?

  • CATT: one week
  • Immuno trypanolysis: 3 weeks
  • Microscopic examination: one to two weeks depending on the number of tests.
  • PCR: one week
  • ELISA: one week

For CATT test and microscopic examination urgent testing is possible. An extra costs of 10% will be charged.

Accredited

CATT serum & microscopic examination
Certificate 147-TEST

Laatste wijziging op: 18/11/2019