Yellow fever virus neutralizing antibodies

Which test do we use?

PRNT90 (Plaque-reduction neutralization test)

Sampling instructions

For 1 or 2 serological analyses: at least 1 ml serum
For 3 to 5 serological analyses: at least 2 ml serum

Storage conditions

The serum can be sent at room temperature, but the duration of transportation must be reduced as much as possible (avoid weekends or holidays).

Important advice

Checking status after vaccination is unnecessary is most people, for more information see

If neutralising antibodies are requested, the date (year) of the last yellow fever vaccination needs to be mentioned on the request form.


Request form National Reference Center (NRC)


For request in the context of symptoms, see ‘Yellow fever virus antibodies’


Once per week

Reference values

protected >1/10

not protected <1/10

How soon can you expect results?

Depends on the total number of requested analyses. See ‘How frequently do we perform this analysis’.





Laatste wijziging op: 23/01/2020