Japanese encephalitis virus PCR

Which test do we use?

Real-time RT-PCR for detection of Japanese encephalitis virus (JEV)

Sampling instructions

Serum/plasma or cerebrospinal fluid in a sterile tube.


1 ml serum/plasma or 350µl cerebrospinal fluid.


For stable RNA concentrations in the serum, the serum preferably has to be separated from the cells immediately after collection. If not possible, the sample can be stored for 6 hours at most at 4°C.

Storage conditions

Store samples at 4°C until transportation. Samples can be sent at room temperature, but transportation time must be kept to a minimum (avoid weekends and holidays). Avoid repeated freezing and thawing of the samples.

Important advice

This test is only carried out on samples obtained at most 7 days after the onset of the fever. PCR is complementary to tracing antibodies against the virus. Mention the country of origin, the date symptoms started and the date of sample collection on the request form.


Request form National Reference Center (NRC)


1x / week

Reference values


Delivery time of the result

On average: 4 days



ISO 15189 – Belac 147-MED





Laatste wijziging op: 13/07/2023