Treponema pallidum (serological confirmation)

Which test do we use?

For corfirmations of a treponemal test with a second treponemal test, TPPA is standard.

In exceptional cases a Line Immunoassay (LIA) IgG can be performed.

Sampling instructions

For 1 or 2 serological analyses: at least 1 ml serum
For 3 to 5 serological analyses: at least 2 ml serum

Storage conditions

The serum can be sent at room temperature, but the duration of transportation must be reduced as much as possible (avoid weekends or holidays).

Important advice

The LIA will only be performed after consulting the clinical biologist.


LIA: 1x / 2 weeks

TPPA: 2x / week

Reference values


Delivery time of the result

On average:

LIA: 7 days

TPPA: 3 days


LIA: no


TPPA:   22590-4

IgG:      47238-1

IgM:     47237-3

Laatste wijziging op: 17/01/2023