Mycobacterium tuberculosis complex molecular resistance determination

Which test do we use?

  • Rifampicine Standaard:
    • Xpert MTB/RIF
    • Upon request: LineProbeAssay
    • Upon request in cas of discordant results: rpoB sequencing
  • Isoniazide
    •  LineProbeAssay
    • katG en/of inhA sequencing
  • Ofloxacine
    •  LineProbeAssay
    • gyrAB sequencing
  • Kanamycine
    •  LineProbeAssay
    • rrs and/or eis sequencing
  • Pyrazinamide
    • pncA sequencing

Sampling instructions

  • Xpert MTB/RIF
    • Possible from sputum, bronchiale lavage, bronchiaal aspirate, cerebrospinal fluid, biopsy, pus, punction liquid and positive cultures (solid or liquid medium)
  • Line Probe Assays
    • Possible from sputum, bronchiale lavage, bronchiaal aspirate and positive cultures (solid or liquid medium)
  • Sequencing
    • Possible from sputum, bronchiale lavage, bronchiaal aspirate and positive cultures (solid or liquid medium)

Storage conditions

  • The clinical samples must be kept in the refrigerator at 2-8°C.
  • The cultures are preferably stored at room temperature or specific incubation temperature.
  • The samples and cultures can be sent at room temperature.

Important advice

  • Requests for molecular analysis need to be mentioned explicitly on the request form.
  • This analysis is only performed if multi-drug resistant M. tuberculosisis is suspected.
  • To send cultures on solid medium, the fluid needs to be removed aseptically from the tubes!
  • If you also request a culture and drug-susceptibility testing, the samples cannot be older than 7 days.
  • If you do not need a culture and drug-susceptibility testing, you can also send a DNA extract. Please provide us also with your negative DNA extraction control in that case and mention the extraction method used.
  • For clinical samples, the presence of acid fast bacilli is highly recommended.

Frequence

Daily

How soon can you expect results?

  • Xpert MTB/RIF: within 2 working days
  • in-huis PCR + sequencing (DNA-extractions are intiated on Mondays only):
    • When delivered on Friday (or Thursday if Friday is a holiday): approximately 4 working days.
    • When delivered on any other day: approximately 7 working da
  • LPA: approximately 5 working days

Accredited

Xpert MTB/RIF: yes

Sequencing rpoB: yes

ISO 15189 – Belac 147-MED

Sequencing other genes: no

LPA: requested

Laatste wijziging op: 06/09/2019